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Widal test
 
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Widal test explained in detail.
widal test
 
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Views: 15862 Ravi's Microbiology
Widal test
 
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The video describes the WIDAL test. It talks about the test’s principle, equipment, precautions, testing procedure and interpretation of results.
Views: 10535 LabsforLifeProject
Widal Test - What You Need to Know
 
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Widal test is used for the diagnosis of typhoid fever. Enteric or typhoid fever is a Salmonella typhi bacterial infection. Understand the symptoms and reasons to perform this particular test. Widal Test rates in India: https://www.medifee.com/tests/widal-test-cost/
Views: 89108 MediFee.com
Widal test
 
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This video discusses the slide and tube method of widal test. I will be discussing about the bacteria, what are the antigens we test and why. I will also discuss the interpretation of the results and when we get false negative or false positive reports
Views: 1187 arunharith
विडाल टेस्ट / टाईफाइड़ widal test  हिंदी में Typhoid Fever फीवर symptoms Treatment
 
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Dr Prabhat Agrawal Associate Professor Hospital Panchkuia Nalband churaha Ashok Nagar Agra These videos are for informational purposes only.Never delay in seeking treatment from your doctor.These videos are not a substitute for professional medical advice and treatment. Each case has an individualized treatment plan
Views: 130243 Dr Ruchika Garg
widal test (slide & tube) procedure
 
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Widal test is still a common way of diagnosing enteric fever cases in several countries. Although tube agglutination is the standard, some manufacturers have adapted it for slide agglutination. This video shows qualitative as well as quantitative test on a kit that can be used for both slide as well as tube agglutination. Procedure displayed here is as per manufacturer's directions. Procedure of tube agglutination test varies across kits. Kits designed exclusively for tube agglutination have different procedures.
Views: 637058 Sridhar Rao
Widal test, introduction and procedure in lab
 
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widal test is done to diagnose typhoid fever. Typhoid fever is caused by salmonella typhi. For more, subscribe my new channel https://www.youtube.com/channel/UCB88x_9Ndp38KLGVYg0yZpw
Views: 74432 Talha Raza
Widal test
 
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The Widal test is a presumptive serological test for enteric fever or undulant fever whereby bacteria causing typhoid fever are mixed with serum containing specific antibodies obtained from an infected individual. In case of Salmonella infections, it is a demonstration of the presence of O-soma false-positive result. Test results need to be interpreted carefully in the light of past history of enteric fever, typhoid vaccination, and the general level of antibodies in the populations in endemic areas of the world. Typhidot is the other test used to ascertain the diagnosis of typhoid fever. As with all serological tests, the rise in antibody levels needed to perform the diagnosis takes 7–14 days, which limits it applicability in early diagnosis. Other means of diagnosing Salmonella typhi (and paratyphi) include cultures of blood, urine and faeces. These organisms produce H2S from thiosulfate and can be easily identified on differential media such as Bismuth sulfite agar. Often 2-mercaptoethanol is added to the Widal test. This agent more easily denatures the IgM class of antibodies, so if a decrease in the titer is seen after using this agent, it means that the contribution of IgM has been removed leaving the IgG component. This differentiation of antibody classes is important; as it allows for the distinction of a recent (IgM) from an old infection (IgG). This video is targeted to blind users. Attribution: Article text available under CC-BY-SA Creative Commons image source in video
Views: 50015 Audiopedia
Introduction to lab values and normal ranges | Health & Medicine | Khan Academy
 
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Find out how health professionals use short-hand for labs and the meaning of normal ranges. Rishi is a pediatric infectious disease physician and works at Khan Academy. Created by Rishi Desai. Watch the next lesson: https://www.khanacademy.org/science/health-and-medicine/lab-values/v/what-s-inside-of-blood?utm_source=YT&utm_medium=Desc&utm_campaign=healthandmedicine Missed the previous lesson? https://www.khanacademy.org/science/health-and-medicine/infectious-diseases/malaria/v/preventing-malaria?utm_source=YT&utm_medium=Desc&utm_campaign=healthandmedicine Health & Medicine on Khan Academy: No organ quite symbolizes love like the heart. One reason may be that your heart helps you live, by moving ~5 liters (1.3 gallons) of blood through almost 100,000 kilometers (62,000 miles) of blood vessels every single minute! It has to do this all day, everyday, without ever taking a vacation! Now that is true love. Learn about how the heart works, how blood flows through the heart, where the blood goes after it leaves the heart, and what your heart is doing when it makes the sound “Lub Dub.” About Khan Academy: Khan Academy is a nonprofit with a mission to provide a free, world-class education for anyone, anywhere. We believe learners of all ages should have unlimited access to free educational content they can master at their own pace. We use intelligent software, deep data analytics and intuitive user interfaces to help students and teachers around the world. Our resources cover preschool through early college education, including math, biology, chemistry, physics, economics, finance, history, grammar and more. We offer free personalized SAT test prep in partnership with the test developer, the College Board. Khan Academy has been translated into dozens of languages, and 100 million people use our platform worldwide every year. For more information, visit www.khanacademy.org, join us on Facebook or follow us on Twitter at @khanacademy. And remember, you can learn anything. For free. For everyone. Forever. #YouCanLearnAnything Subscribe to Khan Academy’s Health & Medicine channel: https://www.youtube.com/channel/UC1RAowgA3q8Gl7exSWJuDEw?sub_confirmation=1 Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
Views: 367291 khanacademymedicine
WIDAL Test For Typhoid Diagnosis
 
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#Ravismicrobiology This video describe Widal test for diagnosis of typhoid fever, it includes both typoid and para typhoid testing by slide agglutination test in this video. Hindi version is also available... 👇 https://youtu.be/zJEWhoCVXw4 ↪ Official Facebook : https://www.facebook.com/ravismicrobiolgy ↪ Official Instagram : https://www.instagram.com/ravismicrobiology ↪ Official Twitter : https://www.twitter.com/@RMicrobiology
Views: 14338 Ravi's Microbiology
What is Widal Test and How Widal Test is done?
 
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Widal test is done to make presumptive diagnosis of Typhoid fever. Widal test is an agglutination test that detects the level of antibodies against two antigens Somatic (O) and Falgellar (H) of Salmonella Typhi. Widal test is done by Slide agglutination method and Tube agglutination method. For more details, visit https://www.1mg.com/labs/test/widal-test-1970 https://www.1mg.com/labs/noida/search?pathology=widal-test-tube-agglutination-1979
Views: 1919 1mg
Dr. Joe Brown- How to Read your Blood work / Labs -FULL DESCRIPTIONS AND HOW TO UNDERSTAND IT
 
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Dr. Joe Brown discusses labs and Blood work (WHITE BLOOD CELLS, ANEMIA, PROTEINS, CHOLESTEROL, KIDNEY AND LIVER FUNCTION, HORMONES) and how to read and understand your blood work. He gives descriptions, definitions, and explanations in detail. Visit: Dr. Joe Brown at http://www.DoctorJoeBrown.com - CANCER SURVIVOR !!! -CLICK SUBSCRIBE TO RECEIVE DR. JOE BROWN'S DAILY INFORMATION VIDEOS
Views: 272525 DrJoe Brown
Widal test procedure
 
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Hello my sweet friends. Kaise hain aap log. Friends aaj maine aap sab ko widal test lagane ka sahi procedure bataya hai. Friends agar aap sab ko meri ye video pasand aaye to like jaroor kare aur shear bhi jaroor kare. Mere channel ko subscribe jaroor karen. Tanki main apne sabhi friends ke liye aur bhi achhi achhi video up lode karun. In last so so thanks friends.
Views: 197446 ravi tiwari
Antistreptolysin O (ASO) test detailed explaination
 
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Antistreptolysin O test is explained in detail.
Widal test kya hai|typhoid fever| hindi/urdu
 
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Widal Test is an agglutination test which detects the presence of serum agglutinins (H and O) in patients serum with typhoid and paratyphoid fever. The Widal test is one method that may be used to help make a presumptive diagnosis of enteric fever, also known as typhoid fever keep watching video for more detail Track: Fareoh - Under Water [NCS Release] Music provided by NoCopyrightSounds. Watch: https://youtu.be/xzyRoshFFaA Free Download / Stream: http://ncs.io/UnderWaterYO
Views: 10586 Dr fun dot com
WIDAL TEST PROCEDURE!! SLIDE AND TUBE METHOD!!
 
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WIDAL TEST PROCEDURE!! SLIDE AND TUBE METHOD SALLMONELLA TYPHY AND SALLMONELLA PERA TYPHY, QUALITATIVE IGGLLUTINATION METHOD, #WIDALTESTPROCEDURE #LABTECHNICIAN #widaltubemethod #widalslidemethod
Views: 14068 LAB TECHNICIAN
Interpretation of the Urinalysis (Part 3) - Microscopy and Summary
 
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The final part of a short series on urinalysis interpretation, including microscopy of casts and crystals.
Views: 140214 Strong Medicine
Procedure of Widal Test
 
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widal test is the test of tyfoyed and paratyphoid fever. . youtube chanel :- Health News Subscribe Link:-https://www.youtube.com/channel/UCTByZLtzsBYkjswyJXBaeJQ Facebook link:-www.facebook.com/Medinewsbd24.com Newspaper link:- www.Medinewsbd24.com Please Subscribe Our Chanel.(https://www.youtube.com/channel/UCTByZLtzsBYkjswyJXBaeJQ) Command your idea and Like Plese. Any objection please Command your idea. Thanks for Seen our vedio..... And Subscribe for new vedio.
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Procedure of Widal  Test
 
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Widal test In 1896 and named after its inventor, Georges-Fernand Widal, is a presumptive serological test for enteric fever or undulant fever whereby bacteria causing typhoid and malaria fever are mixed with a serum containing specific antibodies obtained from an infected individual. In cases of Salmonella infection, it is a demonstration of the presence of O-soma false-positive result. Test results need to be interpreted carefully to account for any history of c fever, typhoid vaccination, and the general level of antibodies in the populations in endemic areas of the world. Typhidot is the other test used to ascertain the diagnosis of typhoid fever. As with all serological tests, the rise in antibody levels needed to perform the diagnosis takes 7–14 days, which limits its applicability in early diagnosis. Other means of diagnosing Salmonella typhi (and paratyphi) include cultures of blood, urine and faeces. These organisms produce H2S from thiosulfate and can be identified easily on differential media such as bismuth sulfite agar. 2-mercaptoethanol is often added to the Widal test. This agent more easily denatures the IgM class of antibodies, so if a decrease in the titer is seen after using this agent, it means that the contribution of IgM has been removed leaving the IgG component. This differentiation of antibody classes is important as it allows for the distinction of a recent (IgM) from an old infection (IgG). The Widal test is positive if TO antigen titer is more than 1:160 in an active infection, or if TH antigen titer is more than 1:160 in past infection or in immunized persons. A single Widal test is of little clinical relevance due to the high number of cross-reacting infections, including malaria. If no other tests (either bacteriologic culture or more specific serology) are available, a fourfold increase in the titer (e.g., from 1:40 to 1:640) in the course of the infection, or a conversion from an IgM reaction to an IgG reaction of at least the same titer, would be consistent with a typhoid infection. A new serological test called the Tubex test is neither superior nor better performing than the Widal test. Therefore, Tubex test is not recommended for diagnosis of typhoid fever.[1] join with us: https://www.facebook.com/mtjmbd email us at: [email protected] watch our other video tutorials: Technique/ Procedure of Widal Test https://www.youtube.com/watch?v=pF28SaGEPjA Operating details of Short Wave Diathamy (SWD) https://www.youtube.com/watch?v=kP3wt49Sig4&t=2s Blood Group Procedure https://www.youtube.com/watch?v=jv2HzC0LFzE Calibration procedure in Automatic Biochemistry analyzer(Dimension) https://www.youtube.com/watch?v=PIjVRlDtIw0&t=2s How can you setup new dr list into CBC Cell Counter https://www.youtube.com/watch?v=bGLBNkPkRx8 How can make a CBC report by using Cell Counter machine https://www.youtube.com/watch?v=N_nni3KKoGQ Procedure of serum Bilirubin (total).new tutorial.using biotrade reagent. https://www.youtube.com/watch?v=GUj_CVWOsLA Procedure of urine culture https://www.youtube.com/watch?v=9I6_zsAvO1Y&t=9s Procedure of Creatinine Test https://www.youtube.com/watch?v=9xCP7pCY0Zw How can you performed ESR in Caretium auto machine https://www.youtube.com/watch?v=_g7Co-pAdv8&t=73s A to Z about the PHYSIOTHERAPY with practical https://www.youtube.com/watch?v=7bWXHihztII&t=4s
WATCH PROCEDURE FOR WIDAL AND TEST RESULT IN NIGERIA LOCAL LABORATORY
 
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Introduction to basic medical laboratory works. On this video you learn how to do pcv and widal test.
Views: 1342 OS TV
Typhoid fever
 
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Typhoid fever explained in detail with treatment.
Views: 19263 EASY MEDICINE
Serial Dilution Method Protocol Step Wise Explanation
 
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Serial Dilution Method: Definition and Procedure Explained http://www.biologyexams4u.com/2013/12/serial-dilution-protocol-pdf.html
Views: 298497 biologyexams4u
widal test slide & tube procedure
 
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widal test slide & tube procedure Immunology 2014
Views: 2100 Lion Roar
Hindi -टाइफाइड बुखार   TYPHOID | CAUSES | SYMPTOMS | COMPLICATION | TREATMENT | MANAGEMENT | HINDI
 
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In this video I explained Typhoid fever. Typhoid fever caused by salmonella typhi or salmonella paratyphi bacteria, This spread by faecal-oral route means by excreta.Excreta may contaminate water may spread this bacteria.Contamination of hand from excreta may spread this bacteria by what you eat or drink.Flies may be spread this bacteria by contact with excreta. (excreta means toilet) Problem is diagnosis of this Fever.Unlike malaria,chikungunia,dengue etc fever their diagnosis is done by clinically with immediate lab report but diagnosis of typhoid takes time.Widal test positive after 1st week. Treatment consist of antibiotic therapy for 2 week.Cephalosporin & fluoroquinolone groupnof medicines used. In some patient may may reoccur within few days of treatment then antibiotics for 4 week suggested. In any disease prevention is easy then treatment so take care about it. Thanking You #beyourdoctor #typhoid #typhoidfever #typhoidtreatment #typhoidsymptoms #typhoidcauses ------------------------------------------ Social Links ----------------------------------------------- Like Us On Facebook : - https://www.facebook.com/beyourdoctor2016/ Email :- [email protected]
Views: 497705 Be Your Doctor
Can positive Widal Test indicate Typhoid without fever? - Dr. Anantharaman Ramakrishnan
 
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The WIDAL test is an outdated test. So it does not have any value in the Indian scenario, whereas most Indians have been exposed to vaccinations for typhoid for typhoid fever in the childhood. So in such cases, WIDAL test is of not much use. Any fever can cause elevation in the WIDAL titres. This is called as an anamnestic reaction. So the generalized increased in antibodies in a person who has fever. In a person who does not have fever, and it is positive no matter whatsoever. So the only way to diagnose. Salmonella infection is a blood culture, stool culture or a urine culture. So that is the way to diagnose salmonella.
Malaria Thick Smear Preparation
 
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The video describes the preparation and interpretation of a malarial thick smear for the detection of malarial parasites.
Views: 6658 LabsforLifeProject
Méthodes de Sero-diagnostic de Widal (SDW)
 
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La méthode de SDW est une méthode exécuté permettant de diagnostiquer la fièvre typhoïde qui est une maladie bactérienne transmise par l'ingestion d'eau et des aliments souillés ou par une personne infecté.
Views: 47 Kamal Deen25
Salmonella typhi Antigen Test
 
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salmonella typhi Antigen Test
Views: 19278 hussam poilt
SALMONELLA TYPHI AND PARATYPHI || STRUCTURE, SYMPTOMS, TREATMENT, LIFE CYCLE || BY PHANINDRA GUPTHA
 
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There are two types of salmonella, which causes typhoid and paratyphoid fever. Brief explanation of the topic is given in the video.
Views: 1879 phanindra gupta
Widal Test for Typhoid and Paratyphoid Procedure | Widal Test in Bangla | Widal Test Procedure
 
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Widal Test for Typhoid and Paratyphoid Procedure | Widal Test in Bangla | Widal Test Procedure........ আমাদের Channel এর সকোল Update ভিডিও পেতে আমাদের Channel টি Subscribe কোরুন। ভিডিও টি দেখার জন্য ধন্যবাদ......... -------------------------- #BanglaPathologyTest #WidalTestBangla #WidalTestProcedure
Views: 102 Logic Bengali
Widal test
 
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Widal test detection of typhoid
Views: 6368 Ravi Kumar
LABORATORY PROCEDURE OF DOING PCV, AND WIDAL TEST IN NIGERIA
 
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This the second introduction for part 2 of medical laboratory work.
Views: 648 OS TV
Salmonella typhi IgG/IgM Test
 
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***Caution: This product is not 510(k) cleared by US FDA and thus is not for sale in the United States.*** INTENDED USE QuickProfile™ S. Typhi IgG/IgM Duo Test is an in vitro qualitative immunochromatographic assay for the rapid detection of anti-S.Typhi antibodies in human whole blood/plasma/serum specimen. The test results are intended to help in the diagnosis of S. Typhi infection. INTRODUCTION Typhoid fever is a life threatening illness caused by the bacterium Salmonella typhi, and was observed by Eberth (1880) in the mesenteric nodes and spleen of fatal cases of typhoid fever. It is common in developing countries where it affects about 12.5 million persons annually. The infection is acquired typically by ingestion. On reaching the gut, the bacilli attach themselves to the epithelial cells of the intestinal villi and penetrate to the lamina and submucosa. They are then phagocytosed there by polymorphs and macrophages. The ability to resist intracellular killing and to multiply within these cells is a measure of their virulence. They enter the mesenteric lymph nodes, where they multiply and, via the thoracic duct, enter the blood stream. A transient bacteremia follows, during which the bacilli are seeded in the liver, gall bladder, spleen, bone marrow, lymph nodes and kidneys, where further multiplication takes place. Towards the end of the incubation period, there occurs a massive bacteremia from these sites, heralding the onset of the clinical symptoms. The symptoms of the illness include high fever, headache, abdominal pain, diarrhea or constipation. A quick and accurate diagnosis of typhoid fever at an early stage is not only important for etiological diagnosis, but also to identify and treat the potential carriers and prevent acute disease outbreaks. QuickProfile ™ S. Typhi Antibody Duo Test takes only 10-20 minutes and requires only a small quantity of sample to perform. It is a easy and specific method for detecting S. typhi infection.
Views: 20259 LumiQuick
#TYPHOIDSYMPTOMS| PREVENTION AND TREATMENT IN TELUGU@wakeup
 
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TYPHOID IS #ENTERICFEVER, IT INFECTED BY #CONTAMINATION THROUGH FOOD AND WATER.ITS CAUSED BY #SALLMONELLA TYPHI #BACTERIUM .IT MAINLY LIVES IN THE SMALL INTESTINE OF MAN,IT MIGRATES TO OTHER ORGANS TROUGH BLOOD.IT CAN BE CONFORMED BY #WIDALTEST. #WAKEUP: Wake up YOUTUBE channel for food fitness and health which describes in Telugu .please subscribe and share. WATCH ON YOUETUBE PAGE WAKE UP CHANNEL https://www.youtube.com/c/wakeupTelevision FACE BOOK PAGE https://www.facebook.com/Wakeup-576436086050562/ Backgrund music information* big thanks for giving us music copyright free music* youtube audio library -~-~~-~~~-~~-~- Please watch: "HOW TO CUT ONIONS DIFFERENT TYPES OF DIFFERENT CURRIES IN TELUGU|VEGETABLE CUTTING @ WAKEUP" https://www.youtube.com/watch?v=ZU3QyAFE21A -~-~~-~~~-~~-~-
Views: 50076 wakeup
Antibody test (quantitative tests)
 
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For more information, log on to- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html Source of all articles published in description is Wikipedia. Thanks to original content developers. Link- http://en.wikipedia.org/wiki/Main_Page This video tutorial talks about different immunological assays and antibody test methods and their importance in clinical biology. It also explains different features of antigen antibody reactions and their role in identifying diseases.
Views: 14272 Shomu's Biology
Animated Principle, Procedure and interpretation of Coomb's test (Direct & Indirect)
 
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A complete animated video for introduction, principle, procedure and interpretation of Coomb's test both direct and indirect. For more, subscribe my new channel https://www.youtube.com/channel/UCB88x_9Ndp38KLGVYg0yZpw
Views: 5446 Talha Raza
Saline Agglutination Test
 
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Megan Brashear, CVT, VTS (ECC), explains the reasoning and demonstrates the process of performing and interpreting a saline agglutination test.
Views: 9771 atdove.org
What is SEROLOGY? What does SEROLOGY mean? SEROLOGY meaning, definition & explanation
 
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BROWSE The Internet EASY way with The Audiopedia owned Lightina Browser Android app. INSTALL NOW - https://play.google.com/store/apps/details?id=com.LightinaBrowser_8083351 What is SEROLOGY? What does SEROLOGY mean? SEROLOGY meaning, definition & explanation. Source: Wikipedia.org article, adapted under https://creativecommons.org/licenses/by-sa/3.0/ license. Serology is the scientific study of serum and other bodily fluids. In practice, the term usually refers to the diagnostic identification of antibodies in the serum. Such antibodies are typically formed in response to an infection (against a given microorganism), against other foreign proteins (in response, for example, to a mismatched blood transfusion), or to one's own proteins (in instances of autoimmune disease). Serological tests may be performed for diagnostic purposes when an infection is suspected, in rheumatic illnesses, and in many other situations, such as checking an individual's blood type. Serology blood tests help to diagnose patients with certain immune deficiencies associated with the lack of antibodies, such as X-linked agammaglobulinemia. In such cases, tests for antibodies will be consistently negative. There are several serology techniques that can be used depending on the antibodies being studied. These include: ELISA, agglutination, precipitation, complement-fixation, and fluorescent antibodies. Some serological tests are not limited to blood serum, but can also be performed on other bodily fluids such as semen and saliva, which have (roughly) similar properties to serum. Serological tests may also be used in forensic serology, specifically for a piece of evidence (e.g., linking a rapist to a semen sample).
Views: 34671 The Audiopedia
C reactive protein
 
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This lecture explains about the c reactive protein levels in human body and about the c reactive protein test to find out the high CRP that might help us to diagnose infection and diseases in our body. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching
Views: 107663 Shomu's Biology
Weil Felix Test
 
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Weil felix Test LIKE US ON FACEBOOK : fb.me/Medsimplified BUY USING AFFILIATE LINKS : AMAZON US--- https://goo.gl/XSJtTx AMAZON India http://goo.gl/QsUhku FLIPKART http://fkrt.it/Wiv8RNNNNN FLIPKART MOBILE APP http://fkrt.it/Wiv8RNNNNN is an agglutination test for the diagnosis of rickettsial infections. It was first described in 1916. By virtue of its long history and of its simplicity, it has been one of the most widely employed tests for rickettsia on a global scale, despite being superseded in many settings by more sensitive and specific diagnostic tests. The basis of the test is the presence of antigenic cross-reactivity between Rickettsia spp. and certain serotypes of non-motile Proteus spp., a phenomenon first published by Edmund Weil and Arthur Felix in 1916.[1] The serum of patients diagnosed with epidemic typhus was found to agglutinate in the presence of bacteria now known as Proteus vulgaris. Ensuing work elucidated that it was in fact the somatic (O) antigen that cross-reacted with anti-rickettsial antibodies, and furthermore, that different Proteus O antigens would cross-react with different species of Rickettsia. Typhus group rickettsiae (Rickettsia prowazekii, R. typhi) react with P. vulgaris OX19, and scrub typhus (Orientia tsutsugamushi) reacts with P. mirabilis OXK. The spotted fever group rickettsiae (R. rickettsii, R. africae, R. japonica, etc.) react with P. vulgaris OX2 and OX19, to varying degrees, depending on the species.[2] The Weil–Felix test suffers from poor sensitivity and specificity, with a recent study showing an overall sensitivity as low as 33% and specificity of 46%.[3] Other studies have had similar findings.[4] As a result, it has largely been supplanted by other methods of serology, including indirect immunofluorescence antibody (IFA) testing, which is the gold standard. However, in resource-limited settings, it still remains an important tool in the diagnosis and identification of public health concerns, such as outbreaks of epidemic typhus. Slide method[edit] On a solid surface (glass slide, tile, card), a small amount (50–100 μL) of the patient’s serum is placed. A single drop of the desired antigen is added, and the resulting suspension is mixed and then rotated for one minute. Visible agglutination is indicative of a positive result, and corresponds roughly to a titer of 1:20. Positive results can be further titrated using the tube method, which is more labour-intensive. Tube method[edit] Using 0.25% phenol saline as a diluent, a series of tubes containing twofold dilutions of patient serum are made with a final volume of 1 mL. A drop of antigen suspension is added to each tube, and the mixture is incubated at 50–55 °C for 4–6 hours. A positive tube would show visible flocculation or granulation, which is accentuated when the tube is gently agitated. The titer corresponds to the most dilute tube in the series that still shows positivity. Generally, a titer of ≥1:320 is considered diagnostic. Subscribe https://www.youtube.com/channel/UCOmrniWfKi-uCD6Oh6fqhgw Watch Again https://youtu.be/ggoKUiEPKE0 -~-~~-~~~-~~-~- CHECK OUT NEWEST VIDEO: "Nucleic acids - DNA and RNA structure " https://www.youtube.com/watch?v=0lZRAShqft0 -~-~~-~~~-~~-~-
Views: 20358 MEDSimplified
Coombs Test Made Simple
 
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LIKE US ON FACEBOOK : fb.me/Medsimplified BUY USING AFFILIATE LINKS : AMAZON US--- https://goo.gl/XSJtTx AMAZON India http://goo.gl/QsUhku FLIPKART http://fkrt.it/Wiv8RNNNNN FLIPKART MOBILE APP http://fkrt.it/Wiv8RNNNNN A Coombs test (also known as Coombs' test, antiglobulin test or AGT) is either of two clinical blood tests used in immunohematology and immunology. The two Coombs tests are the direct Coombs test (DCT, also known as direct antiglobulin test or DAT), and the indirect Coombs test (also known as indirect antiglobulin test or IAT). The direct Coombs test is used to test for autoimmune hemolytic anemia; i.e., a condition of a low count of red blood cells (aka RBCs) caused by immune system lysis or breaking of RBC membranes causing RBC destruction. In certain diseases or conditions, an individual's blood may contain IgG antibodies that can specifically bind to antigens on the RBC surface membrane, and their circulating RBCs can become coated with IgG alloantibodies and/or IgG autoantibodies. Complement proteins may subsequently bind to the bound antibodies and cause RBC destruction.[1] The direct Coombs test is used to detect these antibodies or complement proteins that are bound to the surface of red blood cells; a blood sample is taken and the RBCs are washed (removing the patient's own plasma) and then incubated with anti-human globulin (also known as "Coombs reagent"). If this produces agglutination of RBCs, the direct Coombs test is positive, a visual indication that antibodies (and/or complement proteins) are bound to the surface of red blood cells. The indirect Coombs test is used in prenatal testing of pregnant women and in testing blood prior to a blood transfusion. It detects antibodies against RBCs that are present unbound in the patient's serum. In this case, serum is extracted from the blood sample taken from the patient. Then, the serum is incubated with RBCs of known antigenicity; that is, RBCs with known reference values from other patient blood samples. If agglutination occurs, the indirect Coombs test is positive.[2] The two Coombs tests are based on the fact that anti-human antibodies, which are produced by immunizing non-human species with human serum, will bind to human antibodies, commonly IgG or IgM. Animal anti-human antibodies will also bind to human antibodies that may be fixed onto antigens on the surface of red blood cells (also referred to as RBCs), and in the appropriate test tube conditions this can lead to agglutination of RBCs. The phenomenon of agglutination of RBCs is important here, because the resulting clumping of RBCs can be visualised; when clumping is seen the test is positive and when clumping is not seen the test is negative. Common clinical uses of the Coombs test include the preparation of blood for transfusion in cross-matching, screening for atypical antibodies in the blood plasma of pregnant women as part of antenatal care, and detection of antibodies for the diagnosis of immune-mediated haemolytic anemias. Examples of alloimmune hemolysis[edit] Hemolytic disease of the newborn (also known as HDN or erythroblastosis fetalis) Rh D hemolytic disease of the newborn (also known as Rh disease) ABO hemolytic disease of the newborn (the indirect Coombs test may only be weakly positive) Anti-Kell hemolytic disease of the newborn Rh c hemolytic disease of the newborn Rh E hemolytic disease of the newborn wikipidea https://en.wikipedia.org/wiki/Coombs_test Watch Again https://youtu.be/4MJA8Wzp2XM Subscribe https://www.youtube.com/channel/UCOmrniWfKi-uCD6Oh6fqhgw -~-~~-~~~-~~-~- CHECK OUT NEWEST VIDEO: "Nucleic acids - DNA and RNA structure " https://www.youtube.com/watch?v=0lZRAShqft0 -~-~~-~~~-~~-~-
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Interpretation of positive thinking
 
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In this video I try to explain the interpretation of positive thinking with very powerful story because so many people don't know about the real meaning of positive thinking so this video can help to do something better.
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Serology Meaning
 
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Video shows what serology means. The science that studies the reaction between antigens and antibodies in serum.. The characteristics of the blood serums of a particular disease or organism.. A blood test to detect the presence of antibodies.. Serology Meaning. How to pronounce, definition audio dictionary. How to say serology. Powered by MaryTTS, Wiktionary
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widal mp test by slide method modern pathology.
 
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Widal mp test by slide method chenal modern pathology
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Hepatitis C: CDC Viral Hepatitis Serology Training
 
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This training video covers the different serologic tests and serological diagnosis for acute and chronic Hepatitis C virus (HCV) infection and helps the learner understand the meanings of serologic markers and interpret serologic test results. Comments on this video are allowed in accordance with our comment policy: http://www.cdc.gov/SocialMedia/Tools/CommentPolicy.html This video can also be viewed at http://www.cdc.gov/hepatitis/resources/professionals/training/serology/videos/HCV-CDC_SerologyTraining-2015_low.mp4
TSI Test Part 1
 
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